Abelson Helper Integration Site 1 (AHI1) antibody

Antigen: Abelson Helper Integration Site 1 (AHI1)

Synonyms: ORF1, AHI-1, JBTS3, FLJ14023, FLJ20069, dJ71N10.1, DKFZp686J1653, Ahi-1, D10Bwg0629e, 1700015F03Rik, Ahi1, AHI1

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: ELISA

Catalog no.: ABIN185303

Additional Information

Alternative name: AHI1 / Jouberin

Gene ID: 54806

Immunogen: PTAESEAKVKTC

Description: All names and symbols: AHI1, AHI-1, DKFZp686J1653, FLJ14023, FLJ20069, JBTS3, ORF1, dJ71N10.1, Abelson helper integration site, jouberin, Ahi-1 isoform I, OTTHUMP00000017263, OTTHUMP00000017264, OTTHUMP00000017265, contatins SH3 and WD40 domains,. Official Symbol: AHI1. RefSeq number(s): NP_060121.3

Specificity: Human 1196 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 20kDa band in human brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to NP_060121.3 The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Jiang, Hanna, Kaouass et al.: "Ahi-1, a novel gene encoding a modular protein with WD40-repeat and SH3 domains, is targeted by the Ahi-1 and Mis-2 provirus integrations." in: Journal of virology, Vol. 76, Issue 18, pp. 9046-59, 2002 (PubMed).

Ferland, Eyaid, Collura et al.: "Abnormal cerebellar development and axonal decussation due to mutations in AHI1 in Joubert syndrome." in: Nature genetics, Vol. 36, Issue 9, pp. 1008-13, 2004 (PubMed).

Dixon-Salazar, Silhavy, Marsh et al.: "Mutations in the AHI1 gene, encoding jouberin, cause Joubert syndrome with cortical polymicrogyria." in: American journal of human genetics, Vol. 75, Issue 6, pp. 979-87, 2004 (PubMed).