Leucine-Rich Repeat Kinase 2 (LRRK2) (C-Term), (Internal Region) antibody

Details for Product No. ABIN185309
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Antigen
Synonyms PARK8, RIPK7, ROCO2, AURA17, DARDARIN, Gm927, cI-46, AW561911, 4921513O20Rik, 9330188B09Rik, D630001M17Rik, LRRK2
Epitope
C-Term, Internal Region
(31), (20), (18), (18), (12), (11), (8), (6), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(134), (73), (72), (34), (13), (13), (2)
Host
Goat
(97), (50), (4), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(126), (45), (38), (35), (33), (20), (15), (12), (11), (8), (2), (1), (1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN185309
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
Options
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Availability Available

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Immunogen Peptide with sequence CELAEKMRRTSV, from the internal region (near the C Terminus) of the protein sequence according to NP_940980.3.
Cross-Reactivity Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LRRK2 / PARK8
Background Synonyms: LRRK2, PARK8, ROCO2, FLJ45829, DKFZp434H2111, leucine-rich repeat kinase 2, dardarin, Parkinson disease (autosomal dominant) 8
Gene ID 120892
NCBI Accession NP_940980.3
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: This product has been successfully used in Western blot for human brain as described in the following paper: Sharma et al, Neuropathol Appl Neurobiol. 2011 Jun 23, PMID 21696411. Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Brain (hippocampus) shows staining of neuronal cytoplasm. Recommended concentration: 1-2 µg/mL. This product has been successfully used in immunohistochemistry on human brain: Alegre-Abarrategui J et al., Neuropathol Appl Neurobiol. 2007 Oct 26, PMID: 17971075 Immunoelectron microscopy: This antibody has been successfully used in IEM as described in the following paper: Alegre-Abarrategui et al, Hum Mol Genet. 2009 Nov 1,18(21):4022-34, PMID: 19640926. ICC: This antibody has also been successfully used in ICC for human neurons as described in: Sharma et al, Neuropathol Appl Neurobiol. 2011 Jun 23, PMID 21696411.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Leucine-Rich Repeat Kinase 2 (LRRK2) (C-Term), (Internal Region) antibody ABIN185309 (1.5µg/ml) staining of paraffin embedded Human Hippocampus CA4. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Product cited in: Alegre-Abarrategui, Ansorge, Esiri et al.: "LRRK2 is a component of granular alpha-synuclein pathology in the brainstem of Parkinson's disease." in: Neuropathology and applied neurobiology, Vol. 34, Issue 3, pp. 272-83, 2008 (PubMed).

Alegre-Abarrategui, Christian, Lufino et al.: "LRRK2 regulates autophagic activity and localizes to specific membrane microdomains in a novel human genomic reporter cellular model." in: Human molecular genetics, Vol. 18, Issue 21, pp. 4022-34, 2009 (PubMed).

Sharma, Bandopadhyay, Lashley et al.: "LRRK2 expression in idiopathic and G2019S positive Parkinson's disease subjects: a morphological and quantitative study." in: Neuropathology and applied neurobiology, Vol. 37, Issue 7, pp. 777-90, 2011 (PubMed).

General Paisán-Ruíz, Jain, Evans et al.: "Cloning of the gene containing mutations that cause PARK8-linked Parkinson's disease." in: Neuron, Vol. 44, Issue 4, pp. 595-600, 2004 (PubMed).

Hosts (97), (50), (4), (3), (1)
Reactivities (134), (73), (72), (34), (13), (13), (2)
Applications (126), (45), (38), (35), (33), (20), (15), (12), (11), (8), (2), (1), (1), (1), (1)
Conjugates (7), (7), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (31), (20), (18), (18), (12), (11), (8), (6), (5), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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