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Alcohol Dehydrogenase (ADH) (N-Term) antibody

Details for Product No. ABIN185331, Supplier: Log in to see
Antigen
Epitope
N-Term
1
Reactivity
Human
2
2
2
2
1
1
1
Host
Goat
5
1
Clonality
Polyclonal
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
6
2
1
1
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Immunogen Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1, NP_000659.2, NP_000660.1.
Blocking Peptide Blocking peptide for this product available: ABIN370474
Specificity This antibody is expected to recognise the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of human alcohol dehydrogenase.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ADH
Background Synonyms: pan ADH, ADH1B, ADH2, alcohol dehydrogenase IB (class I), beta polypeptide, ADH, beta subunit, aldehyde reductase, alcohol dehydrogenase 2, alcohol dehydrogenase 2 (class I), beta polypeptide, alcohol dehydrogenase 1B (class I), beta polypeptide
Gene ID 124, 125
NCBI Accession NP_000659, NP_000658, NP_000660
Research Area Signaling, Metabolism
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 38 kDa band observed in Human Liver lysates (calculated MW of 39.9 kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 1-3 µg/mL.
IHC: Customer found this product to work in IHC on Human Liver.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Alcohol Dehydrogenase (ADH) (N-Term) antibody (ABIN185331) ABIN185331 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). P...
Background publications Das, Devi, Pal et al.: "Effect of complex formation between Zn2+ ions and the anticancer drug mithramycin upon enzymatic activity of zinc(II)-dependent alcohol dehydrogenase." in: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, Vol. 10, Issue 1, pp. 25-32, 2005 (PubMed).

Huang, Lin, Ko et al.: "Possible interaction of alcohol dehydrogenase and aldehyde dehydrogenase genes with the dopamine D2 receptor gene in anxiety-depressive alcohol dependence." in: Alcoholism, clinical and experimental research, Vol. 28, Issue 3, pp. 374-84, 2004 (PubMed).

Osier, Pakstis, Kidd et al.: "Linkage disequilibrium at the ADH2 and ADH3 loci and risk of alcoholism." in: American journal of human genetics, Vol. 64, Issue 4, pp. 1147-57, 2000 (PubMed).

Ikuta, Fujiyoshi, Kurachi et al.: "Molecular cloning of a full-length cDNA for human alcohol dehydrogenase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 9, pp. 2703-7, 1985 (PubMed).