Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3) antibody

Antigen: Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3)

Synonyms: DD3, DDX, HAKRB, HAKRe, HA1753, HSD17B5, hluPGFS, KIAA0119, AKR1C3, PGFS, MGC134333, DKFZp469P081

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185345

Additional Information

Alternative name: AKR1C3

Gene ID: 8644

Immunogen: CFASHPNYPYSDEY

Format: Affinity-purified

Description: All names and symbols: AKR1C3, DD3, HAKRB, HAKRe, HA1753, HSD17B5, hluPGFS, KIAA0119, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II), prostaglandin F synthase, dihydrodiol dehydrogenase 3, chlordecone reductase homolog, hydroxysteroid (17-beta) dehydrogenase 5, type IIb 3-alpha hydroxysteroid dehydrogenase, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase. Official Symbol: AKR1C3. RefSeq number(s): NP_003730

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in human liver and human breast lysates (calculated MW of 36.9kDa according to NP_003730). Recommended concentration: 0.01-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology, Endocrine system, Metabolism, Hormones

Restrictions: For Research Use only

Images

ABIN185345 (0.03µg/ml) staining of human breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Iczkowski, Qiu, Qian et al.: "The dual 5-alpha-reductase inhibitor dutasteride induces atrophic changes and decreases relative cancer volume in human prostate." in: Urology, Vol. 65, Issue 1, pp. 76-82, 2005 (PubMed).