Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E) antibody

Antigen: Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E)

Synonyms: SemD, SEMA1, SEMAD, SEMAL, coll-1, Hsema-I, SEMAIII, Hsema-III, MGC133243, SEMA3E, fj37c11, wu:fj37c11, si:ch211-120m13.2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185359

Additional Information

Alternative name: SEMA3E

Gene ID: 9723

Immunogen: C-KPEHYRLPRHTLDS

Description: All names and symbols: SEMA3E, SEMAH, coll-5, M-SEMAH, M-SemaK, KIAA0331, M-sema H, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E. Official Symbol: SEMA3E. RefSeq number(s): NP_036563.1. Gene ontology terms: cell motility, development, extracellular space, membrane, neurogenesis, receptor activity

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx 35kDa and 20kDa in Human Prostate lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 89.2kDa according to NP_036563.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Adrenal Medulla shows granular cytoplasmic staining increasing towards the zona reticularis. Recommended concentration, 2-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185359 (2.5µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Gu, Yoshida, Livet et al.: "Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins." in: Science (New York, N.Y.), Vol. 307, Issue 5707, pp. 265-8, 2005 (PubMed).