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Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody

Details for Product No. ABIN185371, Supplier: Log in to see
Antigen
  • FH3
  • HCHOLA3
  • LDLCQ1
  • NARC-1
  • NARC1
  • PC9
  • AI415265
  • AI747682
  • Narc1
Epitope
C-Term
23
15
12
8
5
5
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human
126
37
34
1
1
1
1
1
1
1
1
Host
Goat
94
41
4
2
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
5
4
4
4
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
102
68
28
20
11
11
11
4
4
3
1
1
1
Supplier
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Immunogen Peptide with sequence CRSRHLAQASQELQ, from the C Terminus of the protein sequence according to NP_777596.2.
Blocking Peptide Blocking peptide for this product available: ABIN369831
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PCSK9 (PCSK9 Antibody Abstract)
Background Synonyms: PCSK9, FH3, NARC1, HCHOLA3, proprotein convertase subtilisin/kexin type 9, neural apoptosis regulated convertase 1, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin/kexin type 9, LDLCQ1, NARC-1
Gene ID 255738
NCBI Accession NP_777596
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75 kDa band observed in Human Adipose and Pancreas lysates (calculated MW of 74.3 kDa according to NP_777596.2). Recommended concentration: 0.3-1 µg/mL. Detects transfected human pcsk9 in McA cell lysates (see figure) as two bands of approx. 73 and 60 kDa, representing unprocessed and processed protein. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1,14(9):1161-9, PMID: 15772090. Data obtained from antibodies from a previous goat. An anonymous customer found positive results in WB on Human Liver.
IHC: In paraffin embedded Human Kidney shows ER and nuclear membrane staining in PCT. Recommended concentration: 2-6 µg/mL. Data obtained from antibodies from a previous goat.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody (ABIN185371) ABIN185371 staining (0.2 µg/mL) of McA cell lysates: untransfected (1), transfected w...
 image for anti-Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody (ABIN185371) ABIN185371 (0.3 µg/mL) staining of Human Adipose lysate (35 µg protein in RIPA buffer...
 image for anti-Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody (ABIN185371) ABIN185371 (4 µg/mL) staining of paraffin embedded Human Kidney. Steamed antigen retr...
Background publications Lalanne, Lambert, Amar et al.: "Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells." in: Journal of lipid research, Vol. 46, Issue 6, pp. 1312-9, 2005 (PubMed).

Cohen, Pertsemlidis, Kotowski et al.: "Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9." in: Nature genetics, Vol. 37, Issue 2, pp. 161-5, 2005 (PubMed).