Hepatocyte Nuclear Factor 4 alpha (HNF4A) antibody

Antigen: Hepatocyte Nuclear Factor 4 alpha (HNF4A)

Synonyms: TCF, HNF4, MODY, MODY1, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, FLJ39654, HNF4alpha, HNF4A

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185378

Additional Information

Alternative name: HNF4A

Gene ID: 3172

Immunogen: RLSKTLVDMDMADY-C

Format: Affinity-purified

Description: All names and symbols: HNF4A, TCF, HNF4, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, hepatocyte nuclear factor 4, alpha, HNF4-alpha, transcription factor-14. Official Symbol: HNF4A. RefSeq number(s): NP_849180.1, NP_000448.3, NP_849181.1. Gene ontology terms: RNA polymerase II transcription factor activity, blood coagulation, lipid metabolism, nucleus, regulation of transcription from Pol II promoter, steroid binding, steroid hormone receptor activity, transcription factor activity,

Specificity: This antibody is expected to recognise the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 52kDa and 48kDa bands observed in lysates of Human hepatocellular carcinoma cell line HepG2 (calculated MW of 52.8kDa according to NP_000448.3 and 46.6kDa according to NP_849181.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185378 (0.1µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Damcott, Hoppman, Ott et al.: "Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish." in: Diabetes, Vol. 53, Issue 12, pp. 3337-41, 2004 (PubMed).