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Arrestin 3 antibody (Arrestin, beta 2) (Internal Region)

Details for Product anti-ARRB2 Antibody No. ABIN185395, Supplier: Log in to see
Antigen
  • AI326910
  • ARB2
  • arb2
  • arr2
  • ARR2
  • arrb2
  • ARRB2
  • arrestin
  • AW122872
  • barr2
  • BARR2
  • BARRES
  • betaarr2
  • zgc:64007
Epitope
Internal Region
13
8
7
5
4
3
3
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
81
22
15
5
5
4
2
1
1
1
1
1
1
Host
Goat
51
18
12
Clonality
Polyclonal
Conjugate
This Arrestin 3 antibody is un-conjugated
2
2
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
71
34
34
21
19
11
3
2
2
1
1
Supplier
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Immunogen Peptide with sequence C-HDHIPLPRPQS, from the internal region of the protein sequence according to NP_004304.1, NP_945355.1.
Blocking Peptide Blocking peptide for this product available: ABIN369151
Specificity This antibody is expected to recognise both reported isoforms NP_004304.1 and NP_945355.1.
Cross-Reactivity (Details) No crossreactivity is expected with Arrestin beta 1.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Arrestin beta 2 (ARRB2 Antibody Abstract)
Background Synonyms: arrestin, beta 2, ARRB2, HGNC:712, ARB2, ARR2, DKFZp686L0365, arrestin beta 2, beta-arrestin 2, BARR2
Molecular Weight 46.1kDa
Gene ID 409
NCBI Accession NP_004304, NP_945355
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 40 kDa band observed in Human Brain lysates (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1 µg/mL. An anonymous customer found positive results in WB on Rat aortic smooth muscle. An anonymous customer found positive results in WB on Mouse smooth muscle. An anonymous customer found positive results in WB on Mouse aortic smooth muscle. An anonymous customer found positive results in WB on Human colon adenocarcinoma cells (SW480).
IHC: In paraffin embedded Human Cerebellum shows mainly nuclear staining of purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration: 4-6 µg/mL. Immunoprecipitation: This antibody has been successfully used in IP from rat leukocytes as described in the following paper: Zakrzewicz et al, Immunobiology. 2011 Jul,216(7):854-61, PMID: 21193245.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Arrestin 3 antibody (Arrestin, beta 2) (Internal Region) (ABIN185395) ABIN185395 (0.03µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer)....
Product cited in: Zakrzewicz, Krasteva, Wilhelm, Dietrich, Wilker, Padberg, Wygrecka, Grau: "Reduced expression of arrestin beta 2 by graft monocytes during acute rejection of rat kidneys." in: Immunobiology, Vol. 216, Issue 7, pp. 854-61, 2011

Napolitano, Pasqualetti, Usiello, Santini, Pacini, Sciamanna, Errico, Tassone, Di Dato, Martella, Cuomo, Fisone, Bernardi, Mandolesi, Mercuri, Standaert, Pisani: "Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism in a model of DYT1 dystonia." in: Neurobiology of disease, Vol. 38, Issue 3, pp. 434-45, 2010

Background publications Lefkowitz, Shenoy: "Transduction of receptor signals by beta-arrestins." in: Science (New York, N.Y.), Vol. 308, Issue 5721, pp. 512-7, 2005