Programmed Cell Death 1 (PDCD1) antibody

Antigen: Programmed Cell Death 1 (PDCD1)

Synonyms: PD1, CD279, SLEB2, hPD-1, hPD-l

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185400

Additional Information

Alternative name: PDCD1

Gene ID: 5133

Immunogen: C-GELDFQWREKTPE

Format: Affinity-purified

Description: All names and symbols: PDCD1 , programmed cell death 1 , HGNC:8760, PD1, SLEB2, hPD-1, hPD-l, programmed death 1. Official Symbol: PDCD1 . RefSeq number(s): NP_005009.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 28-30kDa band observed in Human Bone Marrow lysates (calculated MW of 31.7kDa according to NP_005009.1). Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer

Restrictions: For Research Use only

Images

ABIN185400 (0.6µg/ml) staining of Human Bone Marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Sanghera, Manzi, Bontempo et al.: "Role of an intronic polymorphism in the PDCD1 gene with the risk of sporadic systemic lupus erythematosus and the occurrence of antiphospholipid antibodies." in: Human genetics, Vol. 115, Issue 5, pp. 393-8, 2004 (PubMed).

Ferreiros-Vidal, Gomez-Reino, Barros et al.: "Association of PDCD1 with susceptibility to systemic lupus erythematosus: evidence of population-specific effects." in: Arthritis and rheumatism, Vol. 50, Issue 8, pp. 2590-7, 2004 (PubMed).

Nielsen, Laustrup, Voss et al.: "A putative regulatory polymorphism in PD-1 is associated with nephropathy in a population-based cohort of systemic lupus erythematosus patients." in: Lupus, Vol. 13, Issue 7, pp. 510-6, 2004 (PubMed).

Sheppard, Fitz, Lee et al.: "PD-1 inhibits T-cell receptor induced phosphorylation of the ZAP70/CD3zeta signalosome and downstream signaling to PKCtheta." in: FEBS letters, Vol. 574, Issue 1-3, pp. 37-41, 2004 (PubMed).