ADAM Metallopeptidase Domain 33 (ADAM33) antibody

Antigen: ADAM Metallopeptidase Domain 33 (ADAM33)

Synonyms: FLJ35308, FLJ36751, MGC71889, C20orf153, DJ964F7.1, MGC149823, DKFZp434K0521, Adaml, ADAM13, ADAM33

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185401

Additional Information

Alternative name: ADAM33

Gene ID: 80332

Immunogen: CQSRRCRKNAFQEL

Description: All names and symbols: ADAM33, a disintegrin and metalloproteinase domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889 , a disintegrin and metalloprotease 33, disintegrin and reprolysin metalloproteinase family protein, metalloprotease disintegrin. Official Symbol: ADAM33. RefSeq number(s): NP_079496.1

Specificity: This antibody is expected to recognise isoform alpha (NP_079496) and not isoform beta (NP_694882). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: experiments gave bands at approx 80-90kDa and strong ladder from 26 to 38kDa in Human Prostate lysates after 0.3µg/ml antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature with different antibodies (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul,31(1):13-21, PMID: 14742294). This protein has a calculated MW of 87.7kDa according to NP_079496.1. Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Proteolysis / Ubiquitin, Metalloprotease, Extracellular Matrix, Proteases

Restrictions: For Research Use only

Images

ABIN185401 (0.3µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Blakey, Halapi, Bjornsdottir et al.: "Contribution of ADAM33 polymorphisms to the population risk of asthma." in: Thorax, Vol. 60, Issue 4, pp. 274-6, 2005 (PubMed).

Simpson, Maniatis, Jury et al.: "Polymorphisms in a disintegrin and metalloprotease 33 (ADAM33) predict impaired early-life lung function." in: American journal of respiratory and critical care medicine, Vol. 172, Issue 1, pp. 55-60, 2005 (PubMed).