Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) antibody

Antigen: Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP)

Synonyms: APA, CD249, gp160, im:7152184, si:ch211-146m5.2, MGC127440, DKFZp469K101

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185416

Additional Information

Alternative name: Aminopeptidase A

Gene ID: 2028, 13809 (mouse)

Immunogen: Peptide with sequence C-EQYQKTSLAQEKEK, from the internal region of the protein sequence according to NP_001968.3

Format: Affinity-purified

Description: All names and symbols: aminopeptidase A, ENPEP, glutamyl aminopeptidase (aminopeptidase A) , HGNC:3355, APA, gp160, CD249, glutamyl aminopeptidase. Official Symbol: ENPEP. RefSeq number(s): NP_001968.3.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 170kDa band observed in human brain and testis lysates (calculated MW of 109kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May,87(5):2303-9, PMID: 11994381). Recommended concentration: 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Malerba, Pignatti: "A review of asthma genetics: gene expression studies and recent candidates." in: Journal of applied genetics, Vol. 46, Issue 1, pp. 93-104, 2005 (PubMed).