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Pancreatic Polypeptide (PPY) (Internal Region) antibody

Details for Product No. ABIN185420, Supplier: Log in to see
Antigen
  • AV061413
  • IGSPP
  • PNP
  • PP
  • PPY
Epitope
Internal Region
39
15
3
3
3
2
1
1
Reactivity
Human, Mouse (Murine)
109
29
25
6
5
3
2
1
Host
Goat
58
52
22
Clonality
Polyclonal
Conjugate
Un-conjugated
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
59
47
26
25
13
11
5
5
4
2
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.
Blocking Peptide Blocking peptide for this product available: ABIN370335
Cross-Reactivity Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Pancreatic Polypeptide / PPY (PPY Antibody Abstract)
Background Synonyms: PPY, pancreatic polypeptide, HGNC:9327, PNP, pancreatic polypeptide Y
Gene ID 5539
NCBI Accession NP_002713
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 35-38 kDa band in human pancreas lysates after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.5 kDa according to NP_002713.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3 µg/mL. An anonymous customer found positive results in IHC on Mouse pancreas.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Pancreatic Polypeptide (PPY) (Internal Region) antibody (ABIN185420) ABIN185420 (3µg/ml) staining of paraffin embedded human pancreas. Microwaved antigen ...
Background publications Badman, Flier: "The gut and energy balance: visceral allies in the obesity wars." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1909-14, 2005 (PubMed).