Spleen tyrosine Kinase (SYK) (Internal Region) antibody

Details for Product No. ABIN185423, Supplier: Log in to see
Antigen
  • p72-Syk
  • Sykb
  • p72syk
  • Tyrosine-protein kinase SYK
  • spleen tyrosine kinase
  • ksyk
  • SYK
  • Syk
Alternatives
anti-Human Spleen tyrosine Kinase antibody for Blocking Antibody
Epitope
Internal Region
50
31
24
23
21
16
15
13
12
12
11
10
9
9
9
9
8
8
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
360
161
133
13
11
9
8
8
4
3
1
1
Host
Goat
263
90
18
Clonality
Polyclonal
Conjugate
Un-conjugated
14
11
10
8
6
6
3
2
2
2
2
2
2
2
2
2
2
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
293
135
116
74
73
45
27
23
14
8
4
4
3
3
2
2
1
1
1
1
1
Options
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Immunogen Peptide with sequence C-KYLQQNRHVKDKN, from the internal region of the protein sequence according to NP_003168.2, NP_001128524.1.
Blocking Peptide Blocking peptide for this product available: ABIN369396
Specificity This antibody is expected to recognise both reported isoforms (NP_003168.2, NP_001128524.1). Reported pairs of variants represent identical proteins (NP_003168.2 and NP_001167638.1, NP_001128524.1 and NP_001167639.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Spleen tyrosine kinase / SYK (SYK Antibody Abstract)
Background Synonyms: SYK, spleen tyrosine kinase, HGNC:11491, DKFZp313N1010, FLJ25043, FLJ37489, OTTHUMP00000021628, OTTHUMP00000021629
Gene ID 6850, 20963, 25155
NCBI Accession NP_003168, NP_001128524
Pathways Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70 kDa band observed in Human Bone marrow, Lymph Node, and Spleen lysates (calculated MW of 72.1 kDa according to NP_003168.2). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Spleen tyrosine Kinase (SYK) (Internal Region) antibody (ABIN185423) ABIN185423 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Pr...
Background publications Rogers, Slack, Edwards, Nolte, Schulz, Schweighoffer, Williams, Gordon, Tybulewicz, Brown, Reis e Sousa: "Syk-dependent cytokine induction by Dectin-1 reveals a novel pattern recognition pathway for C type lectins." in: Immunity, Vol. 22, Issue 4, pp. 507-17, 2005 (PubMed).

Underhill, Rossnagle, Lowell, Simmons: "Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production." in: Blood, Vol. 106, Issue 7, pp. 2543-50, 2005 (PubMed).