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Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (Internal Region) antibody

Details for Product No. ABIN185435, Supplier: Log in to see
Antigen
  • KLRK1
  • NKG2D
  • CD314
  • D12S2489E
  • KLR
  • NKG2-D
  • D6H12S2489E
  • Nkg2d
  • Nkrp2
Epitope
Internal Region
13
9
4
2
1
1
1
1
Reactivity
Human
129
54
18
2
2
2
Host
Goat
83
32
21
15
13
2
Clonality
Polyclonal
Conjugate
Un-conjugated
22
17
12
6
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
111
41
40
25
22
20
18
11
4
3
3
3
3
2
2
2
1
1
Supplier
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Immunogen Peptide with sequence C-KVYSKEDQDLLK, from the internal region of the protein sequence according to NP_031386.2.
Blocking Peptide Blocking peptide for this product available: ABIN369392
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NKG2D / KLRK1 (KLRK1 Antibody Abstract)
Background Synonyms: KLRK1, NKG2-D, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NK cell receptor D, NKG2-D type II integral membrane protein, CD314, D12S2489E, FLJ17759, FLJ75772, NKG2D
Gene ID 22914
NCBI Accession NP_031386
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35 kDa band observed in human lymph node and human spleen lysates (calculated MW of 25.3 kDa according to NP_031386.1). The observed molecular weight is explained by glycosylation (Han et al, Blood. 2004 Nov 1,104(9):2858-66. Epub 2004 Jul 6, PMID: 15238421). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (Internal Region) antibody (ABIN185435) ABIN185435 staining (0.3µg/ml) of human spleen lysate (RIPA buffer, 35µg total protei...
Background publications Hayakawa, Smyth: "Innate immune recognition and suppression of tumors." in: Advances in cancer research, Vol. 95, pp. 293-322, 2006 (PubMed).

Blower, Nachury, Heald et al.: "A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly." in: Cell, Vol. 121, Issue 2, pp. 223-34, 2005 (PubMed).