POU Class 5 Homeobox 1 (POU5F1) (C-Term) antibody

Details for Product No. ABIN185438
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Antigen
Synonyms
POU5F1L, POU5F1, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, NF-A3, Oct-3/4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, w ... show more
POU5F1L, POU5F1, OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, NF-A3, Oct-3/4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4, gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, wu:fd18d06 show less
Epitope
C-Term
(29), (22), (17), (10), (7), (7), (6), (6), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(216), (74), (33), (18), (17), (17), (8), (7), (4), (3), (2), (2), (1), (1)
Host
Goat
(151), (69), (16), (9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (6), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(179), (84), (45), (35), (32), (31), (23), (17), (16), (10), (10), (8), (4), (1)
Pubmed 2 references available
Quantity 100 μg
Options
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Catalog No. ABIN185438
374.27 $
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Immunogen Peptide with sequence C-VTTLGSPMHSN, from the C Terminus of the protein sequence according to NP_002692.2, NP_976034.3.
Specificity This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name OCT4 / POU5F1
Background Synonyms: POU5F1, OTF4, POU domain, class 5, transcription factor 1, HGNC:9221, MGC22487, OCT3, OTF3, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3, POU class 5 homeobox 1, DADB-104B20.2, OCT4, POU domain, class 5, transcription factor 1
Gene ID 5460, 18999, 294562
NCBI Accession NP_976034, NP_002692
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 38 kDa band observed in Human Liver lysates (calculated MW of 38.6 kDa according to NP_002692.2). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-POU Class 5 Homeobox 1 (POU5F1) (C-Term) antibody ABIN185438 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Jones, Ulbright, Eble et al.: "OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma." in: The American journal of surgical pathology, Vol. 28, Issue 7, pp. 935-40, 2004 (PubMed).

Chew, Loh, Zhang et al.: "Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells." in: Molecular and cellular biology, Vol. 25, Issue 14, pp. 6031-46, 2005 (PubMed).

Validation Images
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