GATA1 antibody (Internal Region)

Catalog No. ABIN185449

Product Details anti-GATA1 Antibody

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: GATA1

Sequence: DAEAYRHSPV FQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DAEAYRHSPVFQ, from the internal region of the protein sequence according to NP_002040.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50-55 kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8 kDa according to NP_002040.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA1 (GATA1 Products)

Synonyms: ERYF1 antibody, GATA-1 antibody, GF-1 antibody, GF1 antibody, NF-E1 antibody, NFE1 antibody, XLANP antibody, XLTDA antibody, XLTT antibody, Gata-1 antibody, Gf-1 antibody, eryf1 antibody, Eryf1 antibody, GATA1 antibody, gata-1 antibody, Gata1 antibody, si:dkeyp-77f7.5 antibody, gata1 antibody, gata1a-A antibody, xGATA-1 antibody, xgata1 antibody, XGATA-1B antibody, gata1b-A antibody, gta1 antibody, zg1 antibody, NF-E1a antibody, GATA binding protein 1 antibody, GATA binding protein 1 (globin transcription factor 1) antibody, amidase antibody, aspartyl/glutamyl-tRNA amidotransferase subunit A antibody, putative amidase antibody, GatA1 antibody, glutamyl-tRNA amidotransferase subunit A antibody, hematopietic transcription factor GATA-1 antibody, GATA binding protein 1b antibody, GATA binding protein 1 L homeolog antibody, GATA binding protein 1 S homeolog antibody, GATA binding protein 1a antibody, erythroid transcription factor antibody, GATA1 antibody, Gata1 antibody, gatA1 antibody, gata-1 antibody, gata1b antibody, gata1.L antibody, gata1.S antibody, gata1a antibody, LOC108348091 antibody

Background: GATA1, GATA binding protein 1 (globin transcription factor 1), HGNC:4170, ERYF1, GF1, NFE1, GATA binding protein 1, GATA-binding protein 1 (globin transcription factor 1), erythrold transcription factor 1, globin transcription factor 1, transcription fact

Gene ID: 2623

NCBI Accession: NP_002040

Pathways: Cellular Response to Molecule of Bacterial Origin