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ENPP1 antibody (Ectonucleotide Pyrophosphatase/phosphodiesterase 1) (C-Term)

Details for Product anti-ENPP1 Antibody No. ABIN185487, Supplier: Log in to see
Antigen
  • ENPP1
  • si:ch211-142e24.2
  • zgc:136238
  • ARHR2
  • M6S1
  • NPP1
  • NPPS
  • PC-1
  • PCA1
  • PDNP1
  • 4833416E15Rik
  • AI428932
  • C76301
  • CD203c
  • E-NPP1
  • Ly-41
  • Npps
  • Pca
  • Pca-1
  • Pdnp1
  • ttw
  • twy
  • Pc1
Epitope
C-Term
31
19
17
16
5
5
5
5
2
1
1
1
1
Reactivity
Human
81
53
31
10
1
1
Host
Goat
84
20
13
5
1
Clonality
Polyclonal
Conjugate
This ENPP1 antibody is un-conjugated
14
11
7
7
6
5
2
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
73
61
16
15
14
13
7
4
3
2
2
Supplier
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Immunogen Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2.
Blocking Peptide Blocking peptide for this product available: ABIN369376
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ENPP1 / PC1 (ENPP1 Antibody Abstract)
Background Synonyms: ENPP1, PC-1, ectonucleotide pyrophosphatase/phosphodiesterase 1, HGNC:3356, M6S1, NPP1, NPPS, PCA1, PDNP1, Ly-41 antigen, OTTHUMP00000043194, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase I/nucleotide pyrophosphatase 1, plasma-cell membrane glycoprotein 1
Gene ID 5167
NCBI Accession NP_006199
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: In transfected HEK293 transiently expressing ENPP1 a band of approx. 100 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 105 kDa according to NP_006199.2. Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Liver shows membranous staining in many hepatocytes. This antibody has also been successfully used on Human Pancreas showing membranous staining of acinar cells. Recommended concentration: 1-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-ENPP1 antibody (Ectonucleotide Pyrophosphatase/phosphodiesterase 1) (C-Term) (ABIN185487) ABIN185487 (1µg/ml) staining of paraffin embedded Human Liver. Microwaved antigen ret...
Product cited in: Lau, Doucet, Stadel et al.: "Enpp1: a potential facilitator of breast cancer bone metastasis." in: PLoS ONE, Vol. 8, Issue 7, pp. e66752, 2013 (PubMed).

Simão, Yadav, Narisawa et al.: "Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7598-609, 2010 (PubMed).

Background publications Meyre, Bouatia-Naji, Tounian et al.: "Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes. ..." in: Nature genetics, Vol. 37, Issue 8, pp. 863-7, 2005 (PubMed).