IDO1 antibody (Internal Region)

Catalog No. ABIN185488

Product Details anti-IDO1 Antibody

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IDO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: INDO / IDO

Sequence: CVLANWKKKD PNK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CVLANWKKKDPNK, from the internal region of the protein sequence according to NP_002155.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Lymph Node, Spleen and Placenta lysates (calculated MW of 45.3 kDa according to NP_002155.1). Recommended concentration: 0.5-1.5 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-IDO1 antibody (ABIN185488)

Mohamed, Aboul-Fotouh, Ibrahim, Shehata, Mansour, Yassin, El-Eraky, Abdel-Tawab et al.: "Effects of pentoxifylline, 7-nitroindazole, and imipramine on tumor necrosis factor-? and indoleamine 2,3-dioxygenase enzyme activity in the hippocampus and frontal cortex of chronic ..." in: Neuropsychiatric disease and treatment, Vol. 9, pp. 697-708, (2013) (PubMed).

Target Details for IDO1

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: IDO1 (IDO1 Products)

Background: IDO1, INDO, indoleamine-pyrrole 2,3 dioxygenase, HGNC:6059, CD107B, IDO, Indoleamine 2,3-dioxygenase, indole 2,3-dioxygenase

Gene ID: 3620

NCBI Accession: NP_002155

Pathways: Activated T Cell Proliferation