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Details for Product No. ABIN185500

SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (Internal Region) antibody

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Antigen
Synonyms mKIAA1625, 4930431E10Rik, RGD1309707
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN185500
Quantity 100 µg
Price
261.61 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-PSEGSLEDEELPAQ, from the internal region of the protein sequence according to NP_065162.1, NP_851994.1.
Specificity This antibody is expected to recognise both reported isoforms (NP_065162.1 and NP_851994.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SMURF1
Background Synonyms: SMURF1, SMAD specific E3 ubiquitin protein ligase 1, HGNC:16807, KIAA1625, E3 ubiquitin ligase SMURF1, Smad ubiquitination regulatory factor 1, Smad-specific E3 ubiquitin ligase 1
Gene ID 57154
NCBI Accession NP_065162.1, NP_851994.1
Research Area Proteolysis / Ubiquitin
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Preliminary experiments in lysates of cell lines HEK293, A431 and HepG2 gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Ozdamar, Bose, Barrios-Rodiles et al.: "Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity." in: Science (New York, N.Y.), Vol. 307, Issue 5715, pp. 1603-9, 2005 (PubMed).

Alternatives for antigen "SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1)", type "Antibodies"
Hosts (36), (7), (4)
Reactivities (42), (30), (15), (13), (12), (12), (12)
Applications (34), (24), (17), (10), (6), (5), (4), (1), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
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