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SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (Internal Region) antibody

Details for Product No. ABIN185500, Supplier: Login to see
Antigen
  • 4930431E10Rik
  • mKIAA1625
  • RGD1309707
Epitope
Internal Region
26
24
17
14
14
10
9
9
4
3
3
2
2
2
1
1
1
1
Reactivity
Human
106
42
22
2
1
1
1
1
1
Host
Goat
93
14
10
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
7
7
7
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
83
76
38
19
11
8
8
5
1
Supplier
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Immunogen Peptide with sequence C-PSEGSLEDEELPAQ, from the internal region of the protein sequence according to NP_065162.1, NP_851994.1.
Blocking Peptide Blocking peptide for this product available: ABIN369582
Specificity This antibody is expected to recognise both reported isoforms (NP_065162.1 and NP_851994.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SMURF1 (SMURF1 Antibody Abstract)
Background Synonyms: SMURF1, SMAD specific E3 ubiquitin protein ligase 1, HGNC:16807, KIAA1625, E3 ubiquitin ligase SMURF1, Smad ubiquitination regulatory factor 1, Smad-specific E3 ubiquitin ligase 1
Gene ID 57154
NCBI Accession NP_065162, NP_851994
Research Area Proteolysis / Ubiquitin
Pathways
Application Notes ELISA: antibody detection limit dilution 1:1000.
Western blot: Preliminary experiments in lysates of cell lines HEK293, A431 and HepG2 gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Ozdamar, Bose, Barrios-Rodiles et al.: "Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity." in: Science (New York, N.Y.), Vol. 307, Issue 5715, pp. 1603-9, 2005 (PubMed).