Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Internal Region) antibody

Details for Product No. ABIN185508
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Antigen
Synonyms ACTFS, HDMX, hdm2, zgc:109834, 1700007J15Rik, AA415488, Mdm-2
Epitope
Internal Region
(41), (23), (20), (17), (16), (11), (9), (8), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(237), (113), (67), (28), (26), (14), (12), (4), (3), (2), (1), (1), (1), (1)
Host
Goat
(232), (22), (4), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (8), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(167), (96), (74), (72), (54), (40), (26), (21), (16), (14), (8), (7), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185508
374.27 $
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Immunogen Peptide with sequence C-DELSGERQRKRHKSD, from the internal region of the protein sequence according to NP_002383.2.
Specificity This antibody is expected to recognize isoform MDM2 (NP_002383.2) only.
Cross-Reactivity Rat (Rattus), Cat (Feline), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MDM2
Background Synonyms: MDM2, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), HGNC:6973, MGC71221, hdm2, mouse double minute 2 homolog, mouse double minute 2, human homolog of, p53-binding protein, p53-binding protein MDM2, ubiquitin-protein ligase E3 Mdm2, Mdm2 p53 binding protein homolog (mouse), HDMX, MGC5370, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, OTTHUMP00000183495, double minute 2, human homolog of
Gene ID 4193
NCBI Accession NP_002383
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 55 kDa band observed in Human Liver lysates (calculated MW of 56.0 kDa according to NP_002383.2). In transfected HEK293 transiently expressing MDM2 bands of approx. 48 kDa, 60 kDa, 70 kDa and 90 kDa are observed. These bands are not observed in the non-transfected HEK293. The 80 kDa band is endogenously epressed in the negative control. Recommended concentration: 0.3-1.0 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Internal Region) antibody ABIN185508 (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Bond, Hu, Bond et al.: "A single nucleotide polymorphism in the MDM2 promoter attenuates the p53 tumor suppressor pathway and accelerates tumor formation in humans." in: Cell, Vol. 119, Issue 5, pp. 591-602, 2004 (PubMed).

Validation Images
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