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V-Ets erythroblastosis Virus E26 Oncogene Homolog (Avian) (ERG) (C-Term) antibody

Details for Product No. ABIN185550, Supplier: Log in to see
Antigen
  • ERG
  • p55
  • erg-3
  • erg_E
  • D030036I24Rik
  • erg
  • erg_A
  • zgc:103429
Epitope
C-Term
22
5
2
2
2
2
1
1
1
1
1
Reactivity
Human
90
29
24
4
3
3
2
1
1
1
1
1
Host
Goat
38
35
18
Clonality
Polyclonal
Conjugate
Un-conjugated
3
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
52
37
31
25
19
10
5
2
1
1
Supplier
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Immunogen Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|.
Blocking Peptide Blocking peptide for this product available: ABIN369567
Specificity This antibody is expected to recognise all reported isoforms (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1)
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ERG (ERG Antibody Abstract)
Background Synonyms: ERG, v-ets erythroblastosis virus E26 oncogene like (avian), HGNC:3446, erg-3, p55, transcriptional regulator ERG (transforming protein ERG), v-ets avian erythroblastosis virus E26 oncogene related, v-ets erythroblastosis virus E26 oncogene like, v-ets erythroblastosis virus E26 oncogene homolog (avian), OTTHUMP00000115970, OTTHUMP00000208036, TMPRSS2-ERG prostate cancer specific, ets-related
Gene ID 2078, 13876, 170909
NCBI Accession NP_001129626, NP_001129627, NP_004440, NP_891548
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Hematopoietic Progenitors
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments in lysates of cell lines HEK293, A431 and Jurkat gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
IHC: In paraffin embedded Human Prostate shows nuclear staining in secretory cells of the glands. Recommended concentration: 4-8 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-V-Ets erythroblastosis Virus E26 Oncogene Homolog (Avian) (ERG) (C-Term) antibody (ABIN185550) ABIN185550 (10µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen re...
Background publications Tomlins, Rhodes, Perner et al.: "Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer." in: Science (New York, N.Y.), Vol. 310, Issue 5748, pp. 644-8, 2005 (PubMed).

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