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IFT88 antibody (C-Term)

Verified IFT88 Reactivity: Human, Mouse WB, IF, ELISA, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185555
  • Target See all IFT88 Antibodies
    IFT88 (Intraflagellar Transport 88 Homolog (IFT88))
    Binding Specificity
    • 11
    • 9
    • 8
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 38
    • 17
    • 7
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 36
    • 2
    Goat
    Clonality
    • 36
    • 2
    Polyclonal
    Conjugate
    • 18
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    This IFT88 antibody is un-conjugated
    Application
    • 38
    • 29
    • 21
    • 17
    • 16
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)
    Purpose
    IFT88 / Polaris
    Sequence
    KKRIDEDDFA DEE
    Specificity
    This antibody is expected to recognise many reported isoforms.
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2, NP_006522.2.
    Isotype
    IgG
  • Application Notes

    Western Blot: Approx 90 kDa band observed in lysates of cell line A549 and in Mouse Testis lysates, and in preliminary testing of Rat Testes lysate (calculated MW of 90.4 kDa according to Hunab NP_001340498.1, and 93.1 kDa according to Mouse NP_033402.2 93.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nucleus and less staining in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HepG2 cells.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Miyoshi, Kasahara, Miyazaki, Shimizu, Taniguchi, Matsuzaki, Tohyama, Asanuma: "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 10, pp. 3289-97, (2009) (PubMed).

    Jiang, Chiou, Wang, Chien, Ho, Tsai, Lin, Tsai, Li: "Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse." in: Human molecular genetics, Vol. 18, Issue 9, pp. 1566-77, (2009) (PubMed).

  • Target
    IFT88 (Intraflagellar Transport 88 Homolog (IFT88))
    Alternative Name
    IFT88 (IFT88 Products)
    Synonyms
    146590_s_at antibody, CG12548 antibody, CG14465 antibody, CG15190 antibody, CG4137 antibody, Dmel\\CG12548 antibody, IFT88 antibody, NOMPB antibody, OSM-5 antibody, Tg737 antibody, nomp antibody, osm-5 antibody, AW552028 antibody, Tg737Rpw antibody, TgN737Rpw antibody, Ttc10 antibody, flexo antibody, fxo antibody, orpk antibody, polaris antibody, D13S1056E antibody, DAF19 antibody, TG737 antibody, TTC10 antibody, hTg737 antibody, intraflagellar transport 88 antibody, intraflagellar transport 88 homolog antibody, intraflagellar transport protein 88 homolog antibody, no mechanoreceptor potential B antibody, IFT88 antibody, ift88 antibody, LOC100518926 antibody, LOC100640298 antibody, LOC100646898 antibody, nompB antibody, Ift88 antibody
    Background
    IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar tra
    Gene ID
    8100, 21821, 305918
    NCBI Accession
    NP_783195, NP_006522, NP_001340507, NP_001340498
    Pathways
    Hedgehog Signaling, Sensory Perception of Sound
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