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EN2 antibody (Engrailed Homeobox 2) (C-Term)

Details for Product anti-EN2 Antibody No. ABIN185576, Supplier: Log in to see
Antigen
  • EN2
  • en
  • en2
  • BB131122
  • En-2
  • RGD1561842
  • Gg-En-2
Epitope
C-Term
53
5
2
1
1
1
Reactivity
Rat (Rattus)
67
26
25
2
1
1
Host
Goat
60
14
4
Clonality
Polyclonal
Conjugate
This EN2 antibody is un-conjugated
6
6
6
5
5
5
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
62
47
11
9
9
4
3
1
1
Supplier
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Immunogen Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.
Blocking Peptide Blocking peptide for this product available: ABIN370319
Cross-Reactivity Human, Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Engrailed-2 (EN2 Antibody Abstract)
Background Synonyms: Engrailed-2, engrailed homolog 2, EN2, AUTS1
Gene ID 2020
NCBI Accession NP_001418
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx. 30 kDa band observed in Mouse and Rat Brain lysates (calculated MW of 34.2 kDa according to Human NP_001418.2 and of 33.8 kDa according to Mouse NP_034264.1 and of 33.7 kDa according to Rat NP_001102684.1). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-EN2 antibody (Engrailed Homeobox 2) (C-Term) (ABIN185576) EB07120 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primar...
Background publications Benayed, Gharani, Rossman et al.: "Support for the homeobox transcription factor gene ENGRAILED 2 as an autism spectrum disorder susceptibility locus." in: American journal of human genetics, Vol. 77, Issue 5, pp. 851-68, 2005 (PubMed).