DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) (C-Term) antibody

Details for Product No. ABIN185580
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Antigen
Synonyms si:ch211-147p17.1, P54H, Xp54, hlr2, me31b, p54, rck, HLR2, P54, RCK, p54h, RGD1564560, 1110001P04Rik, E230023J21Rik, mRCK/P54
Epitope
C-Term
(13), (8), (7), (6), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(44), (10), (9), (3), (1), (1), (1)
Host
Goat
(39), (4), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(44), (22), (13), (8), (4), (4), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185580
374.27 $
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Immunogen Peptide with sequence C-AEYHSEPVEDEKP, from the C Terminus of the protein sequence according to NP_004388.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DEAD-box protein 6
Background Synonyms: DEAD-box protien 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DDX6, HLR2, P54, RCK, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
Gene ID 1656
NCBI Accession NP_004388
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50 kDa band observed in lysates of cell line Jurkat (calculated MW of 53.2 kDa according to NP_NP_004388.1). In transfected HEK293 transiently expressing Human DDX6 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 53 kDa protein is observed in both. Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) (C-Term) antibody ABIN185580 (0.3µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Cougot, Babajko, Séraphin: "Cytoplasmic foci are sites of mRNA decay in human cells." in: The Journal of cell biology, Vol. 165, Issue 1, pp. 31-40, 2004 (PubMed).

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