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DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) (C-Term) antibody

Details for Product No. ABIN185580, Supplier: Login to see
Antigen
  • si:ch211-147p17.1
  • P54H
  • Xp54
  • hlr2
  • me31b
  • p54
  • rck
  • HLR2
  • P54
  • RCK
  • p54h
  • RGD1564560
  • 1110001P04Rik
  • E230023J21Rik
  • mRCK/P54
Epitope
C-Term
35
16
15
13
9
6
5
3
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
97
19
17
6
3
2
1
1
1
1
Host
Goat
71
15
11
Clonality
Polyclonal
Conjugate
Un-conjugated
6
5
5
5
5
5
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
90
53
25
10
6
5
2
2
1
1
Supplier
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Immunogen Peptide with sequence C-AEYHSEPVEDEKP, from the C Terminus of the protein sequence according to NP_004388.1.
Blocking Peptide Blocking peptide for this product available: ABIN368790
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DEAD-box protein 6 (DDX6 Antibody Abstract)
Background Synonyms: DEAD-box protien 6, DEAD (Asp-Glu-Ala-Asp) box polypeptide 6, DDX6, HLR2, P54, RCK, DEAD box-6, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
Gene ID 1656
NCBI Accession NP_004388
Pathways
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50 kDa band observed in lysates of cell line Jurkat (calculated MW of 53.2 kDa according to NP_NP_004388.1). In transfected HEK293 transiently expressing Human DDX6 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 53 kDa protein is observed in both. Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) (C-Term) antibody (ABIN185580) ABIN185580 (0.3µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). P...
Background publications Cougot, Babajko, Séraphin: "Cytoplasmic foci are sites of mRNA decay in human cells." in: The Journal of cell biology, Vol. 165, Issue 1, pp. 31-40, 2004 (PubMed).