Pancreatic and Duodenal Homeobox 1 (PDX1) antibody
| Antigen |
|
| Synonyms |
GSF, IPF1, IUF1, IDX-1, MODY4, PDX-1, STF-1, PDX1, ipf1 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Conjugate
|
Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
|
|
Application
|
Western Blotting (WB) (32), ELISA (31), Immunofluorescence (IF) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (IHC) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)
|
| Catalog no. |
ABIN185605 |
| Quantity |
100 ug |
| Price |
261.61 $ Plus shipping costs $45.00
|
|
Bulk discount
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
IPF1 / PDX1
|
|
Gene ID
|
3651, 18609 (mouse)
|
|
Immunogen
|
C-AAEPEENKRTRTA
|
|
Description
|
All names and symbols: IPF1, insulin promoter factor 1, homeodomain transcription factor , HGNC:6107, IDX-1, IUF1, MODY4, PDX-1, PDX1, STF-1 , insulin upstream factor 1, pancreatic-duodenal homeobox factor 1. Official Symbol: IPF1. RefSeq number(s): NP_000200.1
|
|
Specificity
|
Human, Mouse, Rat, Dog
|
|
Application Notes
|
Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Preliminary experiments in Human Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
|
|
Purification
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Buffer
|
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|
|
Storage
|
Aliquot and store at -20°C. Minimize freezing and thawing.
|
|
Restrictions
|
For Research Use only
|
|
|
Wells, Melton: "Vertebrate endoderm development." in: Annual review of cell and developmental biology, Vol. 15, pp. 393-410, 2000 (PubMed).
|
Alternatives for antigen "Pancreatic and Duodenal Homeobox 1 (PDX1)", type "Antibodies"
|
Hosts
|
Rabbit (49), Mouse (6), Goat (3)
|
|
Reactivities
|
Human (52), Mouse (Murine) (25), Chicken (19), Pig (Porcine) (19), Rat (Rattus) (19), Chimpanzee (1), Cow (Bovine) (1), Dog (Canine) (1)
|
|
Applications
|
Western Blotting (WB) (32), ELISA (31), Immunofluorescence (IF) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (IHC) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (4), Dot Blot (Dot) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)
|
|
Conjugates
|
Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
|
|
Epitopes
|
N-Term (10), C-Term (3), pSer66 (3), pThr11 (3), Thr11 (2), AA 1-284 (1), AA 194-209 (1), AA 198-213 (1), AA 204-284 (1), AA 91-83 (1), Internal Region (1), Ser66 (1)
|