Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibody

Details for Product No. ABIN185607
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Antigen
Synonyms LEF1, 3000002B05, AI451430, Lef-1, LEF-1, TCF10, TCF1ALPHA, TCF7L3
Epitope
Internal Region
(18), (11), (8), (7), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(118), (50), (41), (28), (27), (27), (25), (3), (1), (1)
Host
Goat
(64), (44), (6), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(98), (34), (20), (18), (12), (12), (7), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN185607
374.27 $
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  • +1 404 474 4654
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Immunogen Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LEF1
Background Synonyms: LEF1, lymphoid enhancer-binding factor 1, HGNC:6551, DKFZp586H0919, TCF1ALPHA, lymphoid enhancer binding factor-1
Gene ID 51176, 16842, 161452
NCBI Accession NP_057353
Research Area Transcription Factors, Wnt, Lineage Markers
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). The same band was also observed in Mouse and Rat Spleen lysates. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibody ABIN185607 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Waterman: "Lymphoid enhancer factor/T cell factor expression in colorectal cancer." in: Cancer metastasis reviews, Vol. 23, Issue 1-2, pp. 41-52, 2004 (PubMed).

Radtke, Clevers: "Self-renewal and cancer of the gut: two sides of a coin." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1904-9, 2005 (PubMed).

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