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LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (Internal Region)

Details for Product anti-LEF1 Antibody No. ABIN185607, Supplier: Log in to see
Antigen
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
Alternatives
anti-Human LEF1 antibody for Enzyme Immunoassay
Epitope
Internal Region
21
16
15
7
6
6
6
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
120
35
27
7
4
4
3
2
1
1
1
1
1
Host
Goat
56
45
15
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
2
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
107
61
19
12
5
4
3
3
2
2
1
1
1
Supplier
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Immunogen Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1.
Blocking Peptide Blocking peptide for this product available: ABIN369644
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Synonyms: LEF1, lymphoid enhancer-binding factor 1, HGNC:6551, DKFZp586H0919, TCF1ALPHA, lymphoid enhancer binding factor-1
Gene ID 51176, 16842, 161452
NCBI Accession NP_057353
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). The same band was also observed in Mouse and Rat Spleen lysates. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (Internal Region) (ABIN185607) ABIN185607 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Prim...
Background publications Radtke, Clevers: "Self-renewal and cancer of the gut: two sides of a coin." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1904-9, 2005 (PubMed).

Waterman: "Lymphoid enhancer factor/T cell factor expression in colorectal cancer." in: Cancer metastasis reviews, Vol. 23, Issue 1-2, pp. 41-52, 2004 (PubMed).