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Details for Product No. ABIN185607

Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibody

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Antigen
Synonyms LEF-1, TCF10, TCF1ALPHA, TCF7L3, 3000002B05, AI451430, Lef-1, LEF1
Epitope
»Alternatives Internal Region
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 2 references available
Catalog no. ABIN185607
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1.
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LEF1
Background Synonyms: LEF1, lymphoid enhancer-binding factor 1, HGNC:6551, DKFZp586H0919, TCF1ALPHA, lymphoid enhancer binding factor-1
Gene ID 51176, 16842, 161452
NCBI Accession NP_057353.1
Research Area Transcription Factors, Wnt, Lineage Markers
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). The same band was also observed in Mouse and Rat Spleen lysates. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Waterman: "Lymphoid enhancer factor/T cell factor expression in colorectal cancer." in: Cancer metastasis reviews, Vol. 23, Issue 1-2, pp. 41-52, 2004 (PubMed).

Radtke, Clevers: "Self-renewal and cancer of the gut: two sides of a coin." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1904-9, 2005 (PubMed).

Alternatives for antigen "Lymphoid Enhancer-Binding Factor 1 (LEF1)", type "Antibodies"
Hosts (49), (49), (8), (5)
Reactivities (109), (48), (39), (28), (27), (27), (25), (3), (1), (1)
Applications (90), (36), (20), (18), (11), (7), (6), (2), (1), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (17), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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