Bone Morphogenetic Protein Receptor, Type IA (BMPR1A) antibody

Antigen: Bone Morphogenetic Protein Receptor, Type IA (BMPR1A)

Synonyms: BMPR1A, ALK3, SKR5, CD292, ACVRLK3, 10q23del, Bmpr, BMPR-IA, AU045487, 1110037I22Rik, BRK-1, BMPR-1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185608

Additional Information

Alternative name: BMPR1A

Gene ID: 657

Immunogen: C-KSDSDQKKSEN

Format: Affinity-purified

Description: All names and symbols: BMPR1A, bone morphogenetic protein receptor, type IA , HGNC:1076, ACVRLK3, ALK3, activin A receptor, type II-like kinase 3. Official Symbol: BMPR1A. RefSeq number(s): NP_004320.2

Specificity: Human, Dog 532 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 60kDa band observed in lysates of cell lines HeLa and HepG2 (calculated MW of 60.2kDa according to NP_004320.2). Recommended concentration: 1-3µg/ml. An additional band of 20kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Stem Cells, Hematopoietic Progenitors, Metabolism

Restrictions: For Research Use only

Images

ABIN185608 (1µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Keller, Nickel, Zhang et al.: "Molecular recognition of BMP-2 and BMP receptor IA." in: Nature structural & molecular biology, Vol. 11, Issue 5, pp. 481-8, 2004 (PubMed).