NONO antibody (C-Term)
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- Target See all NONO Antibodies
- NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This NONO antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purpose
- NONO / p54NRB
- Sequence
- NRAAPGAEFA PNK
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-NRAAPGAEFAPNK, from the C Terminus of the protein sequence according to NP_031389.3.
- Isotype
- IgG
- Top Product
- Discover our top product NONO Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Spleen and Testis. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 60 kDa band observed in Mouse Brain lysates (calculated MW of 54.2 kDa according to Human NP_031389.3 and 54.5 kDa according to Mouse NP_075633.2). Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
- Alternative Name
- NONO (NONO Products)
- Synonyms
- NMT55 antibody, NRB54 antibody, P54 antibody, P54NRB antibody, nmt55 antibody, nrb54 antibody, p54nrb antibody, xp54nrb antibody, AA407051 antibody, AV149256 antibody, nonA antibody, non-POU domain containing octamer binding antibody, non-POU domain containing, octamer-binding antibody, non-POU domain containing, octamer binding L homeolog antibody, non-POU-domain-containing, octamer binding protein antibody, NONO antibody, Nono antibody, nono.L antibody
- Background
- NONO, P54NRB , non-POU domain containing, octamer-binding , HGNC:7871, NMT55, NRB54, P54, Nuclear RNA-binding protein, 54-kD, non-POU-domain-containing, octamer-binding, non-Pou domain-containing octamer (ATGCAAAT) binding protein
- Gene ID
- 4841, 53610, 317259
- NCBI Accession
- NP_031389
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