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NONO antibody (C-Term)

Verified NONO Reactivity: Human, Mouse WB, IHC, ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN185629
  • Target See all NONO Antibodies
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    Binding Specificity
    • 15
    • 12
    • 8
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    C-Term
    Reactivity
    • 75
    • 35
    • 32
    • 15
    • 10
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 1
    Human, Mouse
    Host
    • 70
    • 5
    • 1
    Goat
    Clonality
    • 61
    • 15
    Polyclonal
    Conjugate
    • 36
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This NONO antibody is un-conjugated
    Application
    • 58
    • 34
    • 26
    • 17
    • 14
    • 14
    • 14
    • 8
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Purpose
    NONO / p54NRB
    Sequence
    NRAAPGAEFA PNK
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-NRAAPGAEFAPNK, from the C Terminus of the protein sequence according to NP_031389.3.
    Isotype
    IgG
    Top Product
    Discover our top product NONO Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Spleen and Testis. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 60 kDa band observed in Mouse Brain lysates (calculated MW of 54.2 kDa according to Human NP_031389.3 and 54.5 kDa according to Mouse NP_075633.2). Recommended concentration: 0.1-0.3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    Alternative Name
    NONO (NONO Products)
    Synonyms
    NMT55 antibody, NRB54 antibody, P54 antibody, P54NRB antibody, nmt55 antibody, nrb54 antibody, p54nrb antibody, xp54nrb antibody, AA407051 antibody, AV149256 antibody, nonA antibody, non-POU domain containing octamer binding antibody, non-POU domain containing, octamer-binding antibody, non-POU domain containing, octamer binding L homeolog antibody, non-POU-domain-containing, octamer binding protein antibody, NONO antibody, Nono antibody, nono.L antibody
    Background
    NONO, P54NRB , non-POU domain containing, octamer-binding , HGNC:7871, NMT55, NRB54, P54, Nuclear RNA-binding protein, 54-kD, non-POU-domain-containing, octamer-binding, non-Pou domain-containing octamer (ATGCAAAT) binding protein
    Gene ID
    4841, 53610, 317259
    NCBI Accession
    NP_031389
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