S100A7 antibody (Internal Region)

Catalog No. ABIN185647

Product Details anti-S100A7 Antibody

Target: S100A7 (S100 Calcium Binding Protein A7 (S100A7))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This S100A7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Psoriasin / S100A7

Sequence: HKYTRRDDKI DKP

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HKYTRRDDKIDKP, from the internal region of the protein sequence according to NP_002954.1.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 15 kDa band observed in Human Skin lysates (calculated MW of 11.5 kDa according to NP_002954.1). Recommended concentration: 1-3 μg/mL. In transfected HEK293 transiently expressing full-length Human S100A7 (myc and DYKDDDDK tagged), a band of approx. 16 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5 μg/mL. Approx 15 kDa band was also observed in Human Skin lysates (calculated MW of 11.5 kDa according to NP_002954.1). Recommended concentration: 0.3-1 μg/mL.

DS IHC Results: Paraffin embedded Human Tonsil. Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for S100A7

Target: S100A7 (S100 Calcium Binding Protein A7 (S100A7))

Alternative Name: S100A7 (S100A7 Products)

Background: S100A7, S100 calcium binding protein A7 (psoriasin 1) , HGNC:10497, PSOR1, S100A7c , S100 calcium-binding protein A7, S100 calcium-binding protein A7 (psoriasin 1), psoriasin 1, Psoriasin

Gene ID: 6278

NCBI Accession: NP_002954

Pathways: S100 Proteins