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Wilms Tumor 1 (WT1) (N-Term) antibody

Details for Product No. ABIN185674, Supplier: Log in to see
Antigen
  • WT1
  • WT
  • XWT1a
  • wt-1
  • wt1
  • xWT1
  • gud
  • wagr
  • wt33
  • xwt1
  • wit-2
  • AWT1
  • EWS-WT1
  • GUD
  • NPHS4
  • WAGR
  • WIT-2
  • WT33
  • XWT1b
  • XeWT1
  • CWT1
  • WT-1
  • D630046I19Rik
  • Wt-1
  • si:ch211-160j6.3
  • zgc:152697
Epitope
N-Term
22
19
14
13
11
11
9
9
9
5
4
2
1
1
1
1
1
1
1
1
Reactivity
Human
130
34
31
2
1
1
1
1
1
Host
Goat
70
46
14
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
4
3
3
3
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
88
47
45
38
36
26
17
3
3
2
2
1
1
Supplier
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Immunogen Peptide with sequence QDPASTCVPEPASQH, from the N Terminus of the protein sequence according to NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3.
Blocking Peptide Blocking peptide for this product available: ABIN370392
Specificity This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Wilms tumor 1 / WT1 (WT1 Antibody Abstract)
Background Synonyms: WT1, Wilms tumor 1, GUD, WAGR, WIT-2, WT33
Gene ID 7490
NCBI Accession NP_077743, NP_077742, NP_000369, NP_077744
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 55 kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3 kDa according to NP_000369.3). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Wilms Tumor 1 (WT1) (N-Term) antibody (ABIN185674) ABIN185674 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). ...
Background publications Rao, Pham, Imam et al.: "Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis." in: Genes & development, Vol. 20, Issue 2, pp. 147-52, 2006 (PubMed).