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NLRP3 antibody (NLR Family, Pyrin Domain Containing 3) (Internal Region)

Details for Product anti-NLRP3 Antibody No. ABIN185676, Supplier: Log in to see
Antigen
  • CIAS1
  • Nalp3
  • avp
  • cias1
  • nalp3
  • pypaf1
  • agtavprl
  • AGTAVPRL
  • AII
  • AVP
  • C1orf7
  • CLR1.1
  • FCAS
  • FCU
  • MWS
  • NALP3
  • PYPAF1
  • AII/AVP
  • Cias1
  • Mmig1
  • Pypaf1
Epitope
Internal Region
38
29
15
14
6
5
4
3
3
3
2
2
2
2
2
1
1
Reactivity
Human
160
35
29
6
5
4
2
1
1
Host
Goat
86
49
29
3
Clonality
Polyclonal
Conjugate
This NLRP3 antibody is un-conjugated
6
6
6
5
5
5
2
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
104
83
29
26
18
13
9
8
6
4
4
2
1
Supplier
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Immunogen Peptide with sequence C-DLYEKAKRDEPK, from the internal region of the protein sequence according to NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1.
Blocking Peptide Blocking peptide for this product available: ABIN368895
Specificity This antibody is expected to recognise all five reported isoforms (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CIAS1 / Cryopyrin (NLRP3 Antibody Abstract)
Background Synonyms: CIAS1, cold autoinflammatory syndrome 1, AGTAVPRL, AII/AVP, C1orf7, FCAS, FCU, MWS, NALP3, PYPAF1, AII/AVP receptor-like, Muckle-Wells syndrome, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, angiotensin/vasopressin receptor AII/AVP-like, cryopyrin
Gene ID 114548
NCBI Accession NP_899632, NP_001230062, NP_004886, NP_001120934, NP_001120933
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave an approx 70 kDa band in human lymph node and tonsil lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118 kDa according to NP_004886.3 and 106 kDa according to NP_899632.1. The 70 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Kanneganti, Ozören, Body-Malapel et al.: "Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp3." in: Nature, Vol. 440, Issue 7081, pp. 233-6, 2006 (PubMed).