NLRP3 antibody (Internal Region)

Catalog No. ABIN185676

Product Details anti-NLRP3 Antibody

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: CIAS1 / Cryopyrin (Internal)

Sequence: DLYEKAKRDE PK

Specificity: This antibody is expected to recognise all five reported isoforms (NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DLYEKAKRDEPK, from the internal region of the protein sequence according to NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts. Recommended concentration: 2-4 μg/mL.

Western Blot: Preliminary experiments gave an approx 70 kDa band in human lymph node and tonsil lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculat

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3 (NLRP3 Products)

Synonyms: CIAS1 antibody, Nalp3 antibody, avp antibody, cias1 antibody, nalp3 antibody, pypaf1 antibody, agtavprl antibody, AGTAVPRL antibody, AII antibody, AVP antibody, C1orf7 antibody, CLR1.1 antibody, FCAS antibody, FCU antibody, MWS antibody, NALP3 antibody, PYPAF1 antibody, AII/AVP antibody, Cias1 antibody, Mmig1 antibody, Pypaf1 antibody, NLR family pyrin domain containing-like antibody, NLR family pyrin domain containing 3 antibody, NLR family, pyrin domain containing 3 L homeolog antibody, NLR family, pyrin domain containing 3 antibody, NLRPL antibody, NLRP3 antibody, nlrp3.L antibody, Nlrp3 antibody

Background: CIAS1, cold autoinflammatory syndrome 1, AGTAVPRL, AII/AVP, C1orf7, FCAS, FCU, MWS, NALP3, PYPAF1 , AII/AVP receptor-like, Muckle-Wells syndrome, NACHT, LRR and PYD containing protein 3, PYRIN-containing APAF1-like protein 1, angiotensin/vasopressin recep

Gene ID: 114548

NCBI Accession: NP_004886, NP_899632, NP_001230062, NP_001120933, NP_001120934

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome