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Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A) antibody

Antigen

Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A)
Synonyms semF, SEMAF, FLJ12815, Semaf, AI464145, 5930434A13, 9130201M22Rik, SEMA5A, fa95e03, wu:fa95e03
Clonality Polyclonal
Host
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA
1 reference available
Catalog no. ABIN185680
Quantity 100 ug
Price 374.27 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Semaphorin 5A
Gene ID 9037, 20356 (mouse), 310207 (rat)
Immunogen DEATKKACYSKGKS
Description All names and symbols: SEMA5A, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, HGNC:10736, SEMAF, semF, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane. Official Symbol: SEMA5A. RefSeq number(s): NP_003957.1
Specificity Human, Mouse, Rat, Dog

Application Details
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Application Notes Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121kDa according to NP_003957.1. The 90kDa and 25kDa band were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Research Area Neurology
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Maraganore, de Andrade, Lesnick et al.: "High-resolution whole-genome association study of Parkinson disease." in: American journal of human genetics, Vol. 77, Issue 5, pp. 685-93, 2005 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A)", type "Antibodies"
Hosts Rabbit (25), Goat (3), Mouse (1), Sheep (1)
Reactivities Human (28), Mouse (Murine) (21), Cow (Bovine) (19), Dog (Canine) (19), Rat (Rattus) (19), Chicken (18), Horse (Equine) (18), Pig (Porcine) (18), Sheep (Ovine) (18)
Applications Flow Cytometry (FACS) (15), Immunofluorescence (IF) (15), ELISA (14), Western Blotting (WB) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes N-Term (2), C-Term (1), Internal Region (1), Ser106 (1)