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GTF2I Repeat Domain Containing 1 (GTF2IRD1) (Internal Region) antibody

Details for Product No. ABIN185681, Supplier: Log in to see
Antigen
  • GTF2IRD1
  • 1700012P16Rik
  • BEN
  • Cream1
  • ESTM9
  • GTF3
  • Gtf2il
  • MusTRD1
  • Tg(Alb1-Myc)166.8Sst
  • WBSCR11
  • X83320
  • CREAM1
  • MUSTRD1
  • RBAP2
  • WBS
  • WBSCR12
  • hMusTRD1alpha1
  • Gtf3
  • XBEN
  • XWBSCR11
  • cream1
  • gtf3
  • mustrd1
  • rbap2
  • wbs
  • wbscr11
  • wbscr12
Epitope
Internal Region
27
15
10
8
4
4
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Dog (Canine)
85
21
20
7
3
2
2
1
1
1
Host
Goat
57
16
13
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
65
52
23
12
5
4
3
1
1
Supplier
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Immunogen Peptide with sequence C-NKFTKDTTKLEPAS, from the internal region of the protein sequence according to NP_057412.1, NP_005676.2.
Blocking Peptide Blocking peptide for this product available: ABIN369472
Specificity This antibody is expected to recognise both reported isoforms (NP_057412.1 and NP_005676.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name GTF2IRD1 (GTF2IRD1 Antibody Abstract)
Background Synonyms: GTF2IRD1, GTF2I repeat domain containing 1, HGNC:4661, CREAM1, GTF3, MUSTRD1, RBAP2, WBSCR11, WBSCR12, hMusTRD1alpha1, GTF2I repeat domain-containing 1, Williams-Beuren syndrome chromosome region 11, general transcription factor 3, muscle TFII-I repeat
Gene ID 9569, 57080, 246770
NCBI Accession NP_005676, NP_057412
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 30 kDa and 40 kDa in Human Heart lysate after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 106 kDa according to NP_057412.1 and NP_005676.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Tassabehji, Hammond, Karmiloff-Smith et al.: "GTF2IRD1 in craniofacial development of humans and mice." in: Science (New York, N.Y.), Vol. 310, Issue 5751, pp. 1184-7, 2005 (PubMed).