GTF2IRD1 antibody (Internal Region)

Catalog No. ABIN185681

Product Details anti-GTF2IRD1 Antibody

Target: GTF2IRD1 (General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GTF2IRD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: GTF2IRD1

Sequence: NKFTKDTTKL EPAS

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NKFTKDTTKLEPAS, from the internal region of the protein sequence according to NP_057412.1, NP_005676.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 106 kDa according to NP_057412.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GTF2IRD1

Target: GTF2IRD1 (General Transcription Factor II I Repeat Domain-Containing 1 (GTF2IRD1))

Alternative Name: GTF2IRD1 (GTF2IRD1 Products)

Synonyms: Gtf3 antibody, 1700012P16Rik antibody, BEN antibody, Cream1 antibody, ESTM9 antibody, GTF3 antibody, Gtf2il antibody, MusTRD1 antibody, Tg(Alb1-Myc)166.8Sst antibody, WBSCR11 antibody, X83320 antibody, GTF2IRD1 antibody, CREAM1 antibody, MUSTRD1 antibody, RBAP2 antibody, WBS antibody, WBSCR12 antibody, hMusTRD1alpha1 antibody, XBEN antibody, XWBSCR11 antibody, cream1 antibody, gtf3 antibody, mustrd1 antibody, rbap2 antibody, wbs antibody, wbscr11 antibody, wbscr12 antibody, GTF2I repeat domain containing 1 antibody, general transcription factor II I repeat domain-containing 1 antibody, general transcription factor II-I repeat domain-containing protein 1 antibody, GTF2I repeat domain containing 1 S homeolog antibody, GTF2IRD1 antibody, Gtf2ird1 antibody, gtf2ird1 antibody, LOC100461122 antibody, gtf2ird1.S antibody

Background: GTF2IRD1, GTF2I repeat domain containing 1 , HGNC:4661, CREAM1, GTF3, MUSTRD1, RBAP2, WBSCR11, WBSCR12, hMusTRD1alpha1 , GTF2I repeat domain-containing 1, Williams-Beuren syndrome chromosome region 11, general transcription factor 3, muscle TFII-I repeat

Gene ID: 9569, 57080, 246770

NCBI Accession: NP_057412, NP_005676