STAT3 antibody (Internal Region)
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- Target See all STAT3 Antibodies
- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This STAT3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)
- Purpose
- STAT3
- Sequence
- CRPESQEHPE ADP
- Specificity
- This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence CRPESQEHPEADP, from the internal region of the protein sequence according to NP_644805.1, NP_003141.2, NP_998827.1.
- Isotype
- IgG
- Top Product
- Discover our top product STAT3 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 85 kDa band observed in lysates of cell line LNCaP, and approx. 90 kDa in Mouse Heart lysates (calculated MW of 83.1 kDa according to Human NP_998827.1 and 88.1 kDa according to Mouse : NP_998824.1). Recommended concentration: 0.5-3 μg/mL
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
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Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
- Alternative Name
- STAT3 (STAT3 Products)
- Synonyms
- 1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody
- Background
- STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, FLJ20882, MGC16063 , DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3
- Gene ID
- 6774, 20848, 25125
- NCBI Accession
- NP_644805, NP_003141, NP_998827
- Pathways
- JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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