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STAT3 antibody (Internal Region)

Verified STAT3 Reactivity: Human, Mouse WB, IHC, ELISA, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185692
  • Target See all STAT3 Antibodies
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    Binding Specificity
    • 90
    • 54
    • 27
    • 8
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 276
    • 142
    • 135
    • 38
    • 25
    • 25
    • 22
    • 17
    • 17
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Mouse
    Host
    • 228
    • 58
    • 3
    • 2
    • 1
    • 1
    Goat
    Clonality
    • 195
    • 96
    Polyclonal
    Conjugate
    • 163
    • 23
    • 17
    • 10
    • 9
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    This STAT3 antibody is un-conjugated
    Application
    • 217
    • 109
    • 74
    • 57
    • 50
    • 42
    • 41
    • 41
    • 27
    • 24
    • 15
    • 8
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)
    Purpose
    STAT3
    Sequence
    CRPESQEHPE ADP
    Specificity
    This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1).
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence CRPESQEHPEADP, from the internal region of the protein sequence according to NP_644805.1, NP_003141.2, NP_998827.1.
    Isotype
    IgG
    Top Product
    Discover our top product STAT3 Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 85 kDa band observed in lysates of cell line LNCaP, and approx. 90 kDa in Mouse Heart lysates (calculated MW of 83.1 kDa according to Human NP_998827.1 and 88.1 kDa according to Mouse : NP_998824.1). Recommended concentration: 0.5-3 μg/mL

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    Alternative Name
    STAT3 (STAT3 Products)
    Synonyms
    1110034C02Rik antibody, AW109958 antibody, Aprf antibody, APRF antibody, HIES antibody, Xstat3 antibody, aprf antibody, hies antibody, stat3 antibody, wu:fc15d02 antibody, wu:fl59g06 antibody, z-Stat3 antibody, signal transducer and activator of transcription 3 antibody, signal transduction and activation of transcription 3 antibody, signal transducer and activator of transcription 3, gene 1 L homeolog antibody, signal transducer and activator of transcription 3 (acute-phase response factor) antibody, STAT3 antibody, stat3 antibody, Stat3 antibody, stat3.1.L antibody
    Background
    STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, FLJ20882, MGC16063 , DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3
    Gene ID
    6774, 20848, 25125
    NCBI Accession
    NP_644805, NP_003141, NP_998827
    Pathways
    JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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