Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) antibody

Antigen: Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)

Synonyms: APRF, HIES, FLJ20882, MGC16063, fc15d02, fl59g06, z-Stat3, MGC193054, wu:fc15d02, wu:fl59g06, STAT3, DKFZp469E1221

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185692

Additional Information

Alternative name: STAT3

Gene ID: 6774, 20848 (mouse), 25125 (rat)

Immunogen: CRPESQEHPEADP

Format: Affinity-purified

Description: All names and symbols: STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, FLJ20882, MGC16063 , DNA-binding protein APRF, acute-phase response factor, signal transducer and activator of transcription 3. Official Symbol: STAT3. RefSeq number(s): NP_644805.1, NP_003141.2, NP_998827.1

Specificity: This antibody is expected to recognise all three reported isoforms (NP_644805.1, NP_003141.2 and NP_998827.1). Human, Mouse, Rat, Dog 770 aa, 769 aa, 722 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 75kDa band observed in Human Heart and Human Liver lysates (calculated MW of 83.1kDa according to NP_998827.1). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Embryogenesis, Embryonic stem cells, Transcription Factors, Signaling, Receptors, Inflammation

Restrictions: For Research Use only

Images

ABIN185692 (1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Palmqvist, Glover, Hsu et al.: "Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency." in: Stem cells (Dayton, Ohio), Vol. 23, Issue 5, pp. 663-80, 2005 (PubMed).