Dopamine Receptor D4 (DRD4) antibody
| Antigen |
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| Synonyms |
Drd4b, MGC123021, zgc:123021, si:ch211-69h5.1, D4DR, D4R, Drd-4, AW125663, D4RA, DRD4 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
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Application
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Immunofluorescence (IF) (35), Western Blotting (WB) (33), ELISA (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (IHC) (6), Immunocytochemistry (ICC) (5), Immunoelectron Microscopy (IEM) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN185712 |
| Quantity |
100 ug |
| Price |
261.61 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Dopamine receptor D4 / DRD4
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Gene ID
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1815
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Immunogen
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C-PLRYNRQGGSRRQ
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Description
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All names and symbols: DRD4, dopamine receptor D4, D4DR , D(2C) dopamine receptor, dopamine D4 receptor, seven transmembrane helix receptor . Official Symbol: DRD4. RefSeq number(s): NP_000788.2
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Specificity
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Human 419 aa
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 35kDa band in Human Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.9kDa according to NP_000788.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Li, Sham, Owen et al.: "Meta-analysis shows significant association between dopamine system genes and attention deficit hyperactivity disorder (ADHD)." in: Human molecular genetics, Vol. 15, Issue 14, pp. 2276-84, 2006 (PubMed).
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Alternatives for antigen "Dopamine Receptor D4 (DRD4)", type "Antibodies"
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Hosts
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Rabbit (66), Goat (2), Mouse (2)
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Reactivities
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Human (61), Mouse (Murine) (46), Rat (Rattus) (45)
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Applications
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Immunofluorescence (IF) (35), Western Blotting (WB) (33), ELISA (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (IHC) (6), Immunocytochemistry (ICC) (5), Immunoelectron Microscopy (IEM) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
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Epitopes
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C-Term (7), AA 176-185 (5), 3rd Extracellular Domain (3), N-Term (3), Center (2), Cytoplasmic Domain (1), Internal Region (1), Internal Region,AA 365-391 (1), Internal Region,AA 371-400 (1)
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Alternatives 
