Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (Internal Region) antibody

Details for Product No. ABIN185718
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Antigen
Synonyms CG10523, Dmel\\CG10523, Dpark, SD01679, dpk, si:ch211-123f21.1, zgc:112390, LOC100150461, PRKN, Park, Prkn, AR-JP, LPRS2, PDJ, pdr-1
Epitope
Internal Region
(12), (12), (2), (2), (1), (1), (1)
Reactivity
Human
(40), (12), (12), (9), (7)
Host
Goat
(36), (2), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(21), (20), (7), (6), (3), (3), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185718
374.27 $
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Immunogen Peptide with sequence C-DERAAEQARWEAASKE, from the internal region of the protein sequence according to NP_004553.1, NP_054642.1, NP_054643.1.
Specificity This antibody is expected to recognise all reported isoforms (NP_004553.1, NP_054642.1 and NP_054643.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PARK2
Background Synonyms: PARK2, Parkinson disease (autosomal recessive, juvenile) 2, parkin, AR-JP, PDJ, PRKN, E3 ubiquitin ligase, parkin
Gene ID 5071, 50873, 56816
NCBI Accession NP_054642, NP_054643, NP_004553
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 48 kDa band observed in Human Brain (cerebellum and frontal cortex) lysates (calculated MW of 48.7 kDa according to NP_054642.1). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (Internal Region) antibody ABIN185718 (0.1µg/ml) staining of Human Brain (frontal cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Sun, Latourelle, Wooten et al.: "Influence of heterozygosity for parkin mutation on onset age in familial Parkinson disease: the GenePD study." in: Archives of neurology, Vol. 63, Issue 6, pp. 826-32, 2006 (PubMed).

Validation Images
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