Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) antibody

Antigen: Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2)

Synonyms: PDJ, PRKN, AR-JP, LPRS2, SD01679, dpk, Dmel\CG10523, CG10523, Park, Prkn, zgc:112390, PARK2, pdr-1, LOC100150461

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185718

Additional Information

Alternative name: PARK2

Gene ID: 5071, 50873 (mouse), 56816 (rat)

Immunogen: C-DERAAEQARWEAASKE

Description: All names and symbols: PARK2, Parkinson disease (autosomal recessive, juvenile) 2, parkin, AR-JP, PDJ, PRKN , E3 ubiquitin ligase, parkin . Official Symbol: PARK2. RefSeq number(s): NP_004553.1, NP_054642.1, NP_054643.1

Specificity: This antibody is expected to recognise all reported isoforms (NP_004553.1, NP_054642.1 and NP_054643.1). Human, Mouse, Rat, Dog 465 aa, 437 aa, 316 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 48kDa band observed in Human Brain (cerebellum and frontal cortex) lysates (calculated MW of 48.7kDa according to NP_054642.1). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185718 (0.1µg/ml) staining of Human Brain (frontal cortex) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Sun, Latourelle, Wooten et al.: "Influence of heterozygosity for parkin mutation on onset age in familial Parkinson disease: the GenePD study." in: Archives of neurology, Vol. 63, Issue 6, pp. 826-32, 2006 (PubMed).