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Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (Internal Region) antibody

Details for Product No. ABIN185726
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Antigen
Synonyms OTTMUSG00000014964, Pin1-ps1, Pin1l, dod, ubl5, pin1b, pin1, pin1a, DOD, UBL5, 0610025L01Rik, D9Bwg1161e
Epitope
Internal Region
(26), (11), (10), (9), (8), (7), (5), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(125), (62), (61), (28), (26), (13), (2)
Host
Goat
(83), (39), (5), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(96), (62), (27), (20), (19), (16), (10), (9), (4)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185726
374.27 $
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Immunogen Peptide with sequence C-KHSQSRRPSSWRQEKITR, from the internal region of the protein sequence according to NP_006212.1.
Cross-Reactivity Human, Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PIN1
Background Synonyms: PIN1, protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1, DOD, UBL5, peptidyl-prolyl cis/trans isomerase, NIMA-interacting, prolyl isomerase, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
Gene ID 5300, 23988, 298696
NCBI Accession NP_006212.1
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 17 kDa band observed in Rat Brain lysates (calculated MW of 18.2 kDa according to human NP_006212.1 and 18.2 kDa according to mouse NP_075860). Recommended concentration: 0.2-0.6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (Internal Region) antibody ABIN185726 (0.2µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Pastorino, Sun, Lu et al.: "The prolyl isomerase Pin1 regulates amyloid precursor protein processing and amyloid-beta production." in: Nature, Vol. 440, Issue 7083, pp. 528-34, 2006 (PubMed).

Validation Images
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