Aggrecan antibody (AA 588-684)

Catalog No. ABIN1857938

Product Details anti-Aggrecan Antibody

Target: Aggrecan (ACAN)

Binding Specificity: AA 588-684

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aggrecan antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against AGC. It has been selected for its ability to recognize AGC in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: AGC (Glu588-Arg684)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

References for anti-Aggrecan antibody (ABIN1857938)

Chu, Dai, Wang, Tian, Song, Xiao, Shao, Zhang, Zhang: "Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis." in: BMC musculoskeletal disorders, Vol. 18, Issue 1, pp. 78, (2017) (PubMed).

Dai, Chu, Tian, Song, Wang, Zhang, Zhang: "Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized guinea pigs." in: Osteoarthritis and cartilage, Vol. 24, Issue 6, pp. 1103-12, (2016) (PubMed).

Target Details for Aggrecan

Target: Aggrecan (ACAN)

Alternative Name: Aggrecan (AGC) (ACAN Products)

Synonyms: AGC1 antibody, AGCAN antibody, CSPG1 antibody, CSPGCP antibody, MSK16 antibody, SEDK antibody, Agc antibody, Agc1 antibody, Cspg1 antibody, b2b183Clo antibody, cmd antibody, aggrecan antibody, aggrecan core protein antibody, ACAN antibody, Acan antibody, LOC100380783 antibody, AGC antibody

Background: Alternative Names: ACAN, AGC1, AGCAN, CSPG1, CSPCP, CSPGCP, MSK16, SEDK, Cartilage-specific proteoglycan core protein, Large Aggregating Proteoglycan, Chondroitin Sulfate Proteoglycan 1

Pathways: Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport