DEFa3 antibody (AA 21-94)

Catalog No. ABIN1858619

Product Details anti-DEFa3 Antibody

Target: DEFa3 (Defensin, alpha 3, Neutrophil-Specific (DEFa3))

Binding Specificity: AA 21-94

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEFa3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3)

Specificity: The antibody is a rabbit polyclonal antibody raised against DEFa3. It has been selected for its ability to recognize DEFa3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Defensin Alpha 3, Neutrophil Specific (DEFa3) corresdonding to Pro21~Cys94 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for DEFa3

Target: DEFa3 (Defensin, alpha 3, Neutrophil-Specific (DEFa3))

Alternative Name: Defensin Alpha 3, Neutrophil Specific (DEFa3 Products)

Synonyms: Defa27 antibody, Defcr-3 antibody, Defcr3 antibody, DEF3 antibody, HNP-3 antibody, HNP3 antibody, HP-3 antibody, HP3 antibody, defensin, alpha 3, neutrophil-specific antibody, defensin, alpha, 3 antibody, defensin alpha 3 antibody, DEFA3 antibody, Defa3 antibody

Background: HNP3, DEF3, HNP-2, Neutrophil defensin 2

Pathways: Cellular Response to Molecule of Bacterial Origin