DIO2 antibody (AA 30-259)

Catalog No. ABIN1858637

Product Details anti-DIO2 Antibody

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Binding Specificity: AA 30-259

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Deiodinase, Iodothyronine, Type II (DIO2)

Specificity: The antibody is a rabbit polyclonal antibody raised against DIO2. It has been selected for its ability to recognize DIO2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Deiodinase, Iodothyronine, Type II (DIO2) corresdonding to Ser30~Phe259 with Two N-terminal Tags, His-tag and MBP-tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Alternative Name: Deiodinase, Iodothyronine, Type II (DIO2 Products)

Synonyms: 5DII antibody, D2 antibody, DIOII antibody, SelY antibody, TXDI2 antibody, AI324267 antibody, Deio2 antibody, cb684 antibody, DIO2 antibody, dio2-A antibody, 5dii antibody, sely antibody, txdi2 antibody, iodothyronine deiodinase 2 antibody, deiodinase, iodothyronine, type II antibody, deiodinase, iodothyronine, type 2 L homeolog antibody, deiodinase, iodothyronine, type 2 antibody, type II iodothyronine deiodinase antibody, DIO2 antibody, Dio2 antibody, dio2 antibody, dio2.L antibody, LOC100861220 antibody

Background: 5DII, D2, SelY, TXDI2, Thyroxine Deiodinase,Type II, Type-II 5'-deiodinase

Pathways: Hormone Transport, Hormone Activity