ELANE antibody (AA 30-247)

Catalog No. ABIN1858706

Product Details anti-ELANE Antibody

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Binding Specificity: AA 30-247

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELANE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Neutrophil Elastase (NE)

Specificity: The antibody is a rabbit polyclonal antibody raised against NE. It has been selected for its ability to recognize NE in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Neutrophil Elastase (NE) corresdonding to Ile30~Gln247 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-ELANE antibody (ABIN1858706)

Rivera-Franco, Leon-Rodriguez, Torres-Ruiz, Gómez-Martín, Angles-Cano, de la Luz Sevilla-González: "Neutrophil Extracellular Traps Associate with Clinical Stages in Breast Cancer." in: Pathology oncology research : POR, Vol. 26, Issue 3, pp. 1781-1785, (2020) (PubMed).

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: Neutrophil Elastase (ELANE Products)

Synonyms: ELA2 antibody, GE antibody, HLE antibody, HNE antibody, NE antibody, PMN-E antibody, SCN1 antibody, Ela2 antibody, F430011M15Rik antibody, ela2 antibody, ELANE antibody, elastase, neutrophil expressed antibody, proteinase 3 antibody, ELANE antibody, Elane antibody, prtn3 antibody

Background: ELANE, HLE, HNE, ELA2, PMN-E, Neutrophil Elastase, Medullasin, Polymorphonuclear Leukocyte Elastase, Bone Marrow Serine Protease, Human Leukocyte Elastase, PMN Elastase