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EGF antibody (AA 45-275)

EGF Reactivity: Pig WB, IHC, IP, ICC Host: Mouse Monoclonal 2# unconjugated
Catalog No. ABIN1859001
  • Target See all EGF Antibodies
    EGF (Epidermal Growth Factor (EGF))
    Binding Specificity
    • 45
    • 16
    • 11
    • 10
    • 8
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 45-275
    Reactivity
    • 152
    • 85
    • 50
    • 12
    • 11
    • 5
    • 4
    • 1
    • 1
    • 1
    Pig
    Host
    • 217
    • 30
    • 9
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 222
    • 38
    Monoclonal
    Conjugate
    • 115
    • 36
    • 20
    • 14
    • 8
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EGF antibody is un-conjugated
    Application
    • 207
    • 87
    • 62
    • 53
    • 53
    • 29
    • 25
    • 24
    • 17
    • 16
    • 15
    • 14
    • 8
    • 8
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Monoclonal Antibody to Epidermal Growth Factor (EGF)
    Sequence
    MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH
    Specificity
    The antibody is a mouse monoclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.
    Purification
    Protein A + Protein G affinity chromatography
    Immunogen
    Recombinant Epidermal Growth Factor (EGF) corresdonding to Ala45~His275 with N-terminal His Tag
    Clone
    2#
    Isotype
    IgG2b kappa
    Top Product
    Discover our top product EGF Primary Antibody
  • Application Notes

    Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-30 μg/mL Immunocytochemistry: 5-30 μg/mL Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    EGF (Epidermal Growth Factor (EGF))
    Alternative Name
    Epidermal Growth Factor (EGF Products)
    Synonyms
    HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody
    Background
    URG, Beta-Urogastrone, Pro-epidermal growth factor
    Pathways
    NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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