CHI3L1 antibody (AA 112-377) (FITC)

Catalog No. ABIN1859043

Product Details anti-CHI3L1 Antibody (FITC)

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Binding Specificity: AA 112-377

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHI3L1 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-NTQSRRTFI KSVPPFLRTH GFDGLDLAWL YPGRRDKQHF TTLIKEMKAE FIKEAQPGKK QLLLSAALSA GKVTIDSSYD IAKISQHLDF ISIMTYDFHG AWRGTTGHHS PLFRGQEDAS PDRFSNTDYA VGYMLRLGAP ASKLVMGIPT FGRSFTLASS ETGVGAPISG PGIPGRFTKE AGTLAYYEIC DFLRGATVHR ILGQQVPYAT KGNQWVGYDD QESVKSKVQY LKDRQLAGAM VWALDLDDFQ GSFCGQDLRF PLTNAIK

Specificity: It has been selected for its ability to recognize GP39 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant GP39 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against GP39 conjugated to fitc.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant GP39 (Asn112~Lys377) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for CHI3L1

Target: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Alternative Name: Glycoprotein 39, Cartilage (GP39) (CHI3L1 Products)

Synonyms: AW208766 antibody, Brp39 antibody, Gp39 antibody, ASRT7 antibody, CGP-39 antibody, GP-39 antibody, GP39 antibody, HC-gp39 antibody, HCGP-3P antibody, YKL-40 antibody, YKL40 antibody, YYL-40 antibody, hCGP-39 antibody, GP38K antibody, CLP-1 antibody, SPC-40 antibody, SPS-40 antibody, Chil1 antibody, BP40 antibody, MGP-40 antibody, chitinase-like 1 antibody, chitinase 3 like 1 antibody, chitinase 3-like 1 (cartilage glycoprotein-39) antibody, Chil1 antibody, CHI3L1 antibody, Chi3l1 antibody

Target Type: Viral Protein