HEXB antibody (AA 122-311)

Catalog No. ABIN1859140

Product Details anti-HEXB Antibody

Target: HEXB (Hexosaminidase B (Beta Polypeptide) (HEXB))

Binding Specificity: AA 122-311

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HEXB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Hexosaminidase B Beta (HEXb)

Specificity: The antibody is a rabbit polyclonal antibody raised against HEXb. It has been selected for its ability to recognize HEXb in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Hexosaminidase B Beta (HEXb) corresdonding to Thr122~Ser311 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:360-1400 Immunohistochemistry: 5-20 μg/mL

1:36-140 Immunocytochemistry: 5-20 μg/mL

1:36-140 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HEXB

Target: HEXB (Hexosaminidase B (Beta Polypeptide) (HEXB))

Alternative Name: Hexosaminidase B Beta (HEXB Products)

Synonyms: ENC-1AS antibody, hexosaminidase B antibody, hexosaminidase subunit beta antibody, Hexb antibody, HEXB antibody

Background: Hex-B, ENC-1AS, HCC-7, Beta-N-acetylhexosaminidase subunit beta, Cervical cancer proto-oncogene 7 protein, N-acetyl-beta-glucosaminidase subunit beta

Pathways: Sensory Perception of Sound, Glycosaminoglycan Metabolic Process