SLC12A3 antibody (AA 3-146)
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- Target See all SLC12A3 Antibodies
- SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
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Binding Specificity
- AA 3-146
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SLC12A3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Purpose
- Monoclonal Antibody to Na-Cl Cotransporter (NCCT)
- Sequence
- MGHHHHHHSG SEF- ELPTTETP GDATLCSGRF TISTLLSSDE PSPPAAYDSS HPSHLTHSST FCMRTFGYNT IDVVPTYEHY ANSTQPGEPR KVRPTLADLH SFLKQEGRHL HALAFDSRPS HEMTDGLVEG EAGTSSEKNP EEPVRFGWVK GVMIRC
- Specificity
- The antibody is a mouse monoclonal antibody raised against NCCT. It has been selected for its ability to recognize NCCT in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Pig
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Na-Cl Cotransporter (NCCT) corresdonding to Glu3~Cys146 with N-terminal His Tag
- Clone
- C2
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product SLC12A3 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
- Alternative Name
- Na-Cl Cotransporter (SLC12A3 Products)
- Synonyms
- SLC12A3 antibody, slc12a3 antibody, DKFZp469N2315 antibody, NCC antibody, NCCT antibody, TSC antibody, AI035291 antibody, solute carrier family 12 member 3 antibody, solute carrier family 12 (sodium/chloride transporter), member 3 antibody, solute carrier family 12, member 3 antibody, SLC12A3 antibody, slc12a3.2 antibody, Slc12a3 antibody
- Background
- SLC12A3, TSC, NCC, Solute Carrier Family 12 Member 3,Sodium/Chloride Transporters, Thiazide-sensitive sodium-chloride cotransporter, Na-Cl symporter
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