NUP35 antibody (AA 132-310)

Catalog No. ABIN1860083

Product Details anti-NUP35 Antibody

Target: NUP35 (Nucleoporin 35kDa (NUP35))

Binding Specificity: AA 132-310

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP35 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Nucleoporin 35kDa (NUP35)

Specificity: The antibody is a rabbit polyclonal antibody raised against NUP35. It has been selected for its ability to recognize NUP35 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nucleoporin 35kDa (NUP35) corresdonding to Thr132~Lys310 (Accession # Q8NFH5)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:230-900 Immunohistochemistry: 5-20 μg/mL

1:23-90 Immunocytochemistry: 5-20 μg/mL

1:23-90 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NUP35

Target: NUP35 (Nucleoporin 35kDa (NUP35))

Alternative Name: Nucleoporin 35kDa (NUP35 Products)

Synonyms: MGC64281 antibody, nup53 antibody, 2310006I24Rik antibody, 35kDa antibody, 5330402E05Rik antibody, MP44 antibody, NO44 antibody, fj68d11 antibody, wu:fj68d11 antibody, zgc:65979 antibody, NP44 antibody, NUP53 antibody, nucleoporin 35kDa L homeolog antibody, nucleoporin 35 antibody, nucleoporin 35kDa antibody, nucleoporin NUP53 antibody, nup35.L antibody, NUP35 antibody, nup35 antibody, CpipJ_CPIJ009421 antibody, Nup35 antibody

Background: MP44, NP44, Mitotic phosphoprotein 44, Nuclear pore complex protein Nup53