PIAS1 antibody (AA 409-645)

Catalog No. ABIN1860208

Product Details anti-PIAS1 Antibody

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Binding Specificity: AA 409-645

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1)

Specificity: The antibody is a rabbit polyclonal antibody raised against PIAS1. It has been selected for its ability to recognize PIAS1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) corresdonding to Glu409~Pro645 (Accession # O75925)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PIAS1

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Alternative Name: Protein Inhibitor Of Activated STAT 1 (PIAS1 Products)

Synonyms: 2900068C24Rik antibody, Ddxbp1 antibody, GBP antibody, DDXBP1 antibody, GU/RH-II antibody, ZMIZ3 antibody, ddxbp1 antibody, gbp antibody, pias antibody, pias1-A antibody, xPAIS1 antibody, xPIAS1 antibody, zmiz3 antibody, protein inhibitor of activated STAT 1 antibody, protein inhibitor of activated STAT, 1 antibody, protein inhibitor of activated STAT, 1a antibody, protein inhibitor of activated STAT 1 L homeolog antibody, PIAS1 antibody, Pias1 antibody, pias1a antibody, pias1.L antibody

Background: GBP, DDXBP1, GU/RH-II, ZMIZ3, Gu-binding protein, Zinc Finger,MIZ-Type Containing 3, DEAD/H(Asp-Glu-Ala-Asp/His)Box Binding Protein 1, RNA helicase II-binding protein

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C