RORA antibody (AA 164-401)

Catalog No. ABIN1860465

Product Details anti-RORA Antibody

Target: RORA (RAR-Related Orphan Receptor A (RORA))

Binding Specificity: AA 164-401

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RORA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to RAR Related Orphan Receptor Alpha (RORa)

Specificity: The antibody is a rabbit polyclonal antibody raised against RORa. It has been selected for its ability to recognize RORa in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant RAR Related Orphan Receptor Alpha (RORa) corresdonding to Gln164~Met401 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-RORA antibody (ABIN1860465)

Yasui, Matsuzaki, Konno, Hirai: "Global Knockdown of Retinoid-related Orphan Receptor α in Mature Purkinje Cells Reveals Aberrant Cerebellar Phenotypes of Spinocerebellar Ataxia." in: Neuroscience, (2020) (PubMed).

Target Details for RORA

Target: RORA (RAR-Related Orphan Receptor A (RORA))

Alternative Name: RAR Related Orphan Receptor Alpha (RORA Products)

Synonyms: ror1 antibody, ror2 antibody, ror3 antibody, rzra antibody, nr1f1 antibody, MGC146531 antibody, NR1F1 antibody, ROR1 antibody, ROR2 antibody, ROR3 antibody, RZR-ALPHA antibody, RZRA antibody, RORalpha-B antibody, gb:dq017624 antibody, rora2 antibody, 9530021D13Rik antibody, Nr1f1 antibody, nmf267 antibody, sg antibody, staggerer antibody, tmgc26 antibody, RORalpha1 antibody, RAR related orphan receptor A antibody, RAR-related orphan receptor A antibody, RAR-related orphan receptor A, paralog a antibody, RAR-related orphan receptor alpha antibody, RORA antibody, rora antibody, Rora antibody, roraa antibody

Background: ROR1, ROR2, NR1F1, ROR3, RZRA, Nuclear receptor subfamily 1 group F member 1, Retinoid-related orphan receptor-alpha

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha